Two pleosporalean root-colonizing fungi, Fuscosphaeria hungarica gen. et sp. nov. and Delitschia chaetomioides, from a semiarid grassland in Hungary

Abstract In this study, we investigated two unidentified lineages of root-colonizing fungi belonging to the order Pleosporales (Dothideomycetes), which were isolated from Festuca vaginata ( Poaceae ), a dominant grass species in semiarid sandy grasslands Hungary. For molecular phylogenetic studies, seven loci (internal transcribed spacer, partial large subunit and small region nrRNA, transcription elongation factor 1-α, RNA polymerase II largest subunit, second ß-tubulin genes) amplified sequenced. Based on morphology multilocus analyses, found that one lineage belonged Delitschia chaetomioides P. Karst. Delitschiaceae isolates other represented novel monotypic genus family Trematosphaeriaceae (suborder Massarineae). lineage, proposed new genus, Fuscosphaeria, by single species, F. hungarica . both lineages, only immature degenerated sporocarps could be induced. These sterile, black, globose, or depressed globose structures with numerous mycelioid appendages submerged culture media surface autoclaved plant materials. Both are first reported here as fungi.